Organotypic Slice Cultures
Here we describe a technique for studying hippocampal postnatal neurogenesis in the rodent brain using the organotypic slice culture technique.
This method maintains the characteristic topographical morphology of the hippocampus while allowing direct application of pharmacological
agents to the developing hippocampal dentate gyrus. Additionally, slice cultures can be maintained for up to 4 weeks and thus, allow one to study
the maturation process of newborn granule neurons. Slice cultures allow for efficient pharmacological manipulation of hippocampal slices while
excluding complex variables such as uncertainties related to the deep anatomic location of the hippocampus as well as the blood brain barrier.
For these reasons, we sought to optimize organotypic slice cultures specifically for postnatal neurogenesis research.
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Adam (middle) graduated with MSc in 2014. Adam's work is highlighted in Recent Publications
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis